Evaluation of the Replication, Pathogenicity, and Immunogenicity of Avian Paramyxovirus (APMV) Serotypes 2, 3, 4, 5, 7, and 9 in Rhesus Macaques

Khattar, Sunil K.; Nayak, Baibaswata; Kim, Shin-Hee; Xiao, Sa; Samal, Sweety; Paldurai, Anandan; Buchholz, Ursula J.; Collins, Peter L.; Samal, Siba K.

In addition, we examined the serum samples collected on day 28 from rhesus monkeys for reactivity against different APMV proteins in purified virions from egg allantoic fluids by Western blotting assay (Fig. 1) . We did not examine APMV-5 because infectious virus was not recovered from any of the animals, and thus infectivity is minimal or negative. We probed the Western blots with pre-immune and immune serum samples from all the four monkeys infected with each of the other APMVs. Every animal reacted to the respective NP, F 1 , P and M proteins with varying degrees of intensities when 5 mg of each APMV virus preparation were run on the gels and blotted to nitrocellulose. For the HN and F 0 proteins, reactive bands were recognized to varying extents or not at all by sera from individual animals, which is consistent with the known drastic changes in immune reactivity of paramyxovirus HN and F proteins when denatured [50] . All preimmune serum samples were found negative.