Selected article for: "antibody fragment and outer membrane"
Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies
  • Document date: 2013_7_29
    • ID: 06o7pa3d_10
    Snippet: Despite these efforts, most antibody fragments are produced in the periplasm of E. coli using N-terminal leader sequences targeting the periplasmic Sec pathway (51), for example signal peptides derived from outer membrane protein A (OmpA), alkaline phosphatase A (PhoA), or pectate lyase B (PelB) (52-54). Also the SRP pathway can be used for antibody fragment production (55). After expression, recombinant antibodies are usually isolated from the p.....
    Document: Despite these efforts, most antibody fragments are produced in the periplasm of E. coli using N-terminal leader sequences targeting the periplasmic Sec pathway (51), for example signal peptides derived from outer membrane protein A (OmpA), alkaline phosphatase A (PhoA), or pectate lyase B (PelB) (52-54). Also the SRP pathway can be used for antibody fragment production (55). After expression, recombinant antibodies are usually isolated from the periplasmic fraction (56, 57) but also from the culture supernatant (58-61).

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