Author: Barnard, Karen N.; Wasik, Brian R.; LaClair, Justin R.; Buchholz, David W.; Weichert, Wendy S.; Alford-Lawrence, Brynn K.; Aguilar, Hector C.; Parrish, Colin R.
Title: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses Document date: 2019_12_3
ID: 11ejfiwe_24
Snippet: Studies with two strains of IAV and two strains of IBV showed no differences in infection efficiency in WT HEK-293 or MDCK-NBL2 cells compared to their ΔCasD1 or CasD1-OX variants. This is not surprising, since Ͼ95% of the Sia is unacetylated Neu5Ac, which can be utilized by IAV and IBV as a receptor for binding and entry. Although the low levels present on cells were not enough to affect either IAV and IBV infection, we did determine that high.....
Document: Studies with two strains of IAV and two strains of IBV showed no differences in infection efficiency in WT HEK-293 or MDCK-NBL2 cells compared to their ΔCasD1 or CasD1-OX variants. This is not surprising, since Ͼ95% of the Sia is unacetylated Neu5Ac, which can be utilized by IAV and IBV as a receptor for binding and entry. Although the low levels present on cells were not enough to affect either IAV and IBV infection, we did determine that higher levels, such as those found on mouse erythrocytes (ϳ45% O-Ac), were able to block IAV HA binding and decrease IAV NA cleavage efficiency in a strain-dependent manner. It is possible, therefore, that if higher levels are present on secreted proteins in mucus, they could inhibit infection prior to the virus reaching the cell surface. Further analysis of mucus O-acetylation in IAV hosts would be necessary to determine whether 7,9-O-and 9-O-Ac could contribute to the barrier function of mucus in respiratory tissues (41) .
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