Author: Lindqvist, Richard; Mundt, Filip; Gilthorpe, Jonathan D.; Wölfel, Silke; Gekara, Nelson O.; Kröger, Andrea; Överby, Anna K.
Title: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects Document date: 2016_10_24
ID: 09tcnsxv_37
Snippet: The antiviral effect of the supernatant was further investigated using qPCR as a more sensitive method of quantifying viral replication. Sixteen hours before TBEV infection, the cells were treated with either supernatant of 24-h TBEV-infected cells or IFNαB/D. The cells were infected with TBEV, and total RNA was extracted 48 hpi to detect TBEV RNA (Fig. 3g) . Pretreatment with supernatant of infected cells and 5000 U IFNαB/D had a similar inhib.....
Document: The antiviral effect of the supernatant was further investigated using qPCR as a more sensitive method of quantifying viral replication. Sixteen hours before TBEV infection, the cells were treated with either supernatant of 24-h TBEV-infected cells or IFNαB/D. The cells were infected with TBEV, and total RNA was extracted 48 hpi to detect TBEV RNA (Fig. 3g) . Pretreatment with supernatant of infected cells and 5000 U IFNαB/D had a similar inhibitory effect on TBEV replication in WT astrocytes. However, no difference in viral replication was detected in IFNAR −/− astrocytes treated with or without supernatant, indicating a type I IFN-dependent antiviral effect. These results further suggest that the rapid type I IFN response is responsible for limiting viral infection and replication in astrocytes.
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