Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_33
Snippet: Due to strong usage of the cellular metabolism during baculoviral protein expression a high diversity in the post-translational modification was observed. Alternatively to baculoviral expression, insect cells can also be transfected with expression plasmids in a transient or stable manner. Here, usually Schneider 2 (S2) cells of D. melanogaster are used. Secretory production requires a signal sequence like the honeybee melittin leader. Stable tra.....
Document: Due to strong usage of the cellular metabolism during baculoviral protein expression a high diversity in the post-translational modification was observed. Alternatively to baculoviral expression, insect cells can also be transfected with expression plasmids in a transient or stable manner. Here, usually Schneider 2 (S2) cells of D. melanogaster are used. Secretory production requires a signal sequence like the honeybee melittin leader. Stable transfection Frontiers in Immunology | B Cell Biology of Drosophila cell lines with monomeric and dimeric antibody fragments resulted in yields of up to 25 µg/mL (144) .
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