Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_53
Snippet: Besides the transfection or transformation of whole plants or at least organs, monoclonal BY-2 tobacco cell lines that grow in suspension have been developed (219) . Flow cytometric analysis has been used to enrich cells expressing a fluorescent marker which was located on the same T-DNA with the antibody gene. Using this method for the enrichment of high expressing cells, production could be increased up to 13-fold and was shown to be stable for.....
Document: Besides the transfection or transformation of whole plants or at least organs, monoclonal BY-2 tobacco cell lines that grow in suspension have been developed (219) . Flow cytometric analysis has been used to enrich cells expressing a fluorescent marker which was located on the same T-DNA with the antibody gene. Using this method for the enrichment of high expressing cells, production could be increased up to 13-fold and was shown to be stable for 10-12 months.
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