Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_63
Snippet: Insect cells contain a better suited protein folding and secretion apparatus than prokaryotes. Their high robustness combined with less sophisticated requirements for fermentation provide some advantages compared to mammalian cells. However, the development of stable insect cell lines and process technology is not developed as far. Consequently, mammalian cell lines are most widely used for the production of therapeutic antibodies as they provide.....
Document: Insect cells contain a better suited protein folding and secretion apparatus than prokaryotes. Their high robustness combined with less sophisticated requirements for fermentation provide some advantages compared to mammalian cells. However, the development of stable insect cell lines and process technology is not developed as far. Consequently, mammalian cell lines are most widely used for the production of therapeutic antibodies as they provide a sophisticated folding and secretion apparatus as well as human-like glycosylation. For the production of high levels of recombinant antibodies high technical efforts are needed leading to relatively high costs. The maximum reported yield of functional IgG was 5 g/L which cannot be achieved using other expression systems so far, but up-scaling of the production does not lead to a high reduction of the production costs.
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