Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_8
Snippet: Viral DNA was extracted from the cell culture supernatant by using a QIAamp R MinElute R Virus Spin Kit (QIAGEN, Germantown, MD, United States) following the manufacturer's protocols. Viral DNA was subsequently cleaned up and concentrated with AMPureTM XP beads (Beckman Coulter, Indianapolis, IN, United States). The purified DNA was further tested by quantitative PCR for detection of the target virus......
Document: Viral DNA was extracted from the cell culture supernatant by using a QIAamp R MinElute R Virus Spin Kit (QIAGEN, Germantown, MD, United States) following the manufacturer's protocols. Viral DNA was subsequently cleaned up and concentrated with AMPureTM XP beads (Beckman Coulter, Indianapolis, IN, United States). The purified DNA was further tested by quantitative PCR for detection of the target virus.
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