Selected article for: "Applied Biosystems Real time pcr system and Real time pcr"
Author: Lindqvist, Richard; Mundt, Filip; Gilthorpe, Jonathan D.; Wölfel, Silke; Gekara, Nelson O.; Kröger, Andrea; Överby, Anna K.
Title: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects
  • Document date: 2016_10_24
    • ID: 09tcnsxv_12
    Snippet: Total RNA was isolated at the indicated time points using Nucleospin RNA II kit (Macherey-Nagel), and cDNA was synthesized from 200 to 600 ng RNA as previously described [42] . mRNA expression of GAPDH, IFNβ, IFNα2, viperin and tripartite motif 79α (TRIM79α) were detected by QuantiTect primer assay (Qiagen) and the KAPA SYBR FAST qPCR kit (KAPA Biosystems) using a StepOnePlus fast real-time PCR system (Applied Biosystems). TBEV RNA was quanti.....
    Document: Total RNA was isolated at the indicated time points using Nucleospin RNA II kit (Macherey-Nagel), and cDNA was synthesized from 200 to 600 ng RNA as previously described [42] . mRNA expression of GAPDH, IFNβ, IFNα2, viperin and tripartite motif 79α (TRIM79α) were detected by QuantiTect primer assay (Qiagen) and the KAPA SYBR FAST qPCR kit (KAPA Biosystems) using a StepOnePlus fast real-time PCR system (Applied Biosystems). TBEV RNA was quantified using primers previously described [43] and the KAPA PROBE FAST qPCR kit (KAPA Biosystems). Gene expression was normalized to the endogenous GAPDH expression.

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