Ficus septica plant extracts for treating Dengue virus in vitro

Huang, Nan-Chieh; Hung, Wan-Ting; Tsai, Wei-Lun; Lai, Feng-Yi; Lin, You-Sheng; Huang, Mei-Shu; Chen, Jih-Jung; Lin, Wei-Yu; Weng, Jing-Ru; Chang, Tsung-Hsien

In order to understand whether F. septica leaf extracted with methanol, FS-(L)-M, directly inactivated DENV, we preincubated viral stocks of DENV-1, DENV-2 and AiV with a series of dilution doses of FS-(L)-M for 1 h at room temperature. After virus adsorption, the mixture of virus plus the plant extract was replaced by fresh growth medium. At 42 h after infection, the virus-infected cells were analyzed by immunofluorescence assay h at room temperature before used to infect A549 cells (3 × 10 4 ) at MOI = 2.5. After 2 h of virus adsorption, the virus-compound mixture medium was replaced by fresh growth medium for further 42 h incubation. DENV-or AiVinfected cells were detected by immunofluorescence assay. (B) Fluorescence intensity was measured and quantified by the Li-Cor odyssey CLx imaging system. The data are mean ± SD from three independent experiments. *** P < 0.001 by two-tailed Student t test.