Author: Huang, Nan-Chieh; Hung, Wan-Ting; Tsai, Wei-Lun; Lai, Feng-Yi; Lin, You-Sheng; Huang, Mei-Shu; Chen, Jih-Jung; Lin, Wei-Yu; Weng, Jing-Ru; Chang, Tsung-Hsien
Title: Ficus septica plant extracts for treating Dengue virus in vitro Document date: 2017_6_8
ID: 1a3cmi9i_9
Snippet: WST-1 assay (Roche, Basel, Switzerland) was used to monitor cell proliferation (Chou et al., 2014) ; cells were trypsinized and resuspended in culture medium, then plated at 5×10 3 cells per well in 96-well plates and incubated overnight. After plant extracts treatment for 48 h, the cells were incubated with 10 µl WST-1 reagent for 2 h. The cell viability was quantified by multi-well spectrophotometry (Anthos, Biochrom, Cambridge, UK). The abso.....
Document: WST-1 assay (Roche, Basel, Switzerland) was used to monitor cell proliferation (Chou et al., 2014) ; cells were trypsinized and resuspended in culture medium, then plated at 5×10 3 cells per well in 96-well plates and incubated overnight. After plant extracts treatment for 48 h, the cells were incubated with 10 µl WST-1 reagent for 2 h. The cell viability was quantified by multi-well spectrophotometry (Anthos, Biochrom, Cambridge, UK). The absorbance at 450 nm was monitored, and the reference wavelength was set at 620 nm.
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