Author: Funaro, Ada; Gribaudo, Giorgio; Luganini, Anna; Ortolan, Erika; Lo Buono, Nicola; Vicenzi, Elisa; Cassetta, Luca; Landolfo, Santo; Buick, Richard; Falciola, Luca; Murphy, Marianne; Garotta, Gianni; Malavasi, Fabio
Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells Document date: 2008_11_12
ID: 1u2fkwx3_30
Snippet: min at 4°C with anti-human CD23-FITC (Becton Dickinson, Milano, Italy). After washing, fluorescence was analyzed using a FACSCalibur flow cytometer and CellQuest software. CD23 staining was evaluated only on the viable lymphoblast population, gated using forward/orthagonal scatter and PI staining, as described above. Background staining was determined with a FITC-conjugated isotypematched negative control mAb. The number of cells analyzed was 10.....
Document: min at 4°C with anti-human CD23-FITC (Becton Dickinson, Milano, Italy). After washing, fluorescence was analyzed using a FACSCalibur flow cytometer and CellQuest software. CD23 staining was evaluated only on the viable lymphoblast population, gated using forward/orthagonal scatter and PI staining, as described above. Background staining was determined with a FITC-conjugated isotypematched negative control mAb. The number of cells analyzed was 10,000.
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