Author: Lu, Shuai; Chen, Yingzhu; Qin, Kun; Zhou, Jianfang; Lou, Yongliang; Tan, Wenjie
Title: Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China Document date: 2016_4_15
ID: 1ghgd7yg_5
Snippet: Considering the global spread of CCoV and CRCoV infections among dog populations and the possible interspecies transmission from animals to humans, it is necessary to develop rapid and accurate assays for early diagnosis and epidemic control. However, there are very limited studies on CCoV and CRCoV circulating in China, and the antigenic relationship among CCoV, CRCoV and human coronaviruses. Here, the N coding genes of CCoV and CRCoV in China w.....
Document: Considering the global spread of CCoV and CRCoV infections among dog populations and the possible interspecies transmission from animals to humans, it is necessary to develop rapid and accurate assays for early diagnosis and epidemic control. However, there are very limited studies on CCoV and CRCoV circulating in China, and the antigenic relationship among CCoV, CRCoV and human coronaviruses. Here, the N coding genes of CCoV and CRCoV in China were amplified and sequenced, and then the recombinant N proteins of CCoV and CRCoV were prepared and characterized. In addition, cross-reactivity was analyzed between the recombinant proteins and polyclonal mice antisera from human coronaviruses.
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