Genome Annotation Transfer Utility (GATU): rapid annotation of viral genomes using a closely related reference genome

Tcherepanov, Vasily; Ehlers, Angelika; Upton, Chris

erence genome are automatically placed in an Unassigned-ORFs table, which is accessed by clicking on the relevant button below the interactive table. Unassigned-ORFs can be added to the list of selected annotations and the graphical display by checking the Accept box for a given ORF; the Jump button moves the graphical display to show the selected ORF in the window ( Figure 6 ). Several utilities have been built into GATU allowing the user to further investigate the nature of these Unassigned-ORFs. Rightclicking on any of the Unassigned-ORFs and selecting the appropriate option allows the user to view the results of pre-run BLAST searches for the ORF, run BLAST searches if the Manual BLAST search option was checked during the initial annotation run, or initiate a new search using a dif-Results of a NEEDLE alignment run with an Unassigned-ORF; display is presented in the main GATU window Figure 4 Results of a NEEDLE alignment run with an Unassigned-ORF; display is presented in the main GATU window. ferent algorithm (e.g. TBLASTN) or database (e.g. VOCs virus databases [2] , or the NCBI nr database).