Author: Tcherepanov, Vasily; Ehlers, Angelika; Upton, Chris
Title: Genome Annotation Transfer Utility (GATU): rapid annotation of viral genomes using a closely related reference genome Document date: 2006_6_13
ID: 1e2kkhht_30
Snippet: The 1112 and 1482 BLAST searches/NEEDLE alignments required for these two annotation trials took 25 and 40 minutes, respectively, to run on a 1 GHz G4 Macintosh computer. In both cases, a large fraction of the total bacterial ORFs present were automatically annotated correctly by GATU; the majority of the "missed" ORFs were small (<150 nt) hypothetical genes that were either not annotated in the reference genome or were unique to the target genom.....
Document: The 1112 and 1482 BLAST searches/NEEDLE alignments required for these two annotation trials took 25 and 40 minutes, respectively, to run on a 1 GHz G4 Macintosh computer. In both cases, a large fraction of the total bacterial ORFs present were automatically annotated correctly by GATU; the majority of the "missed" ORFs were small (<150 nt) hypothetical genes that were either not annotated in the reference genome or were unique to the target genome. This represents a substantial reduction of the time and effort required for annotation, allowing the annotator to concentrate on those areas of the genome that require expert knowledge to correctly annotate. Figure 6 Genome map panel of GATU interface. Display of an Unassigned-ORF after temporarily selecting Accept box and clicking the Jump button; ORF is shown with green highlighting.
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