Author: Barnard, Karen N.; Wasik, Brian R.; LaClair, Justin R.; Buchholz, David W.; Weichert, Wendy S.; Alford-Lawrence, Brynn K.; Aguilar, Hector C.; Parrish, Colin R.
Title: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses Document date: 2019_12_3
ID: 11ejfiwe_10
Snippet: The low levels of 9-O-and 7,9-O-Ac detected on HEK-293, A549, and MDCK cells by HPLC could be due to the heterogeneity of the population, since this method measures total Sia for all cells in the population. However, it is likely that even on cells expressing higher levels of 7,9-O-and 9-O-Ac, these modified Sia make up a small proportion of the total Sia present in the cell or glycocalyx. Previous studies have reported that podo-planin (GP40 in .....
Document: The low levels of 9-O-and 7,9-O-Ac detected on HEK-293, A549, and MDCK cells by HPLC could be due to the heterogeneity of the population, since this method measures total Sia for all cells in the population. However, it is likely that even on cells expressing higher levels of 7,9-O-and 9-O-Ac, these modified Sia make up a small proportion of the total Sia present in the cell or glycocalyx. Previous studies have reported that podo-planin (GP40 in canine cells) is a primary carrier of 9-O-Ac on MDCK cells and therefore acts as the main receptor for ICV (44, 45) . However, when we costained MDCK cells with an anti-podoplanin antibody and PToV HE-Fc, we saw little correlation between podoplanin and 9-O-Ac staining (Fig. S3) .
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