Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_10
Snippet: A total of 452 ng viral DNA was used for MinION sequencing. The MinION library was constructed with the Rapid Sequencing Kit (SQK-RAD004) according to the manufacturer's instructions. The library was loaded to a SpotON Flow Cell (FLO-MIN106). Then the control software MinKNOW (v3.1.19, downloaded from the ONT website with a custom account) was set up with a run time of 48 h. The basecalling of fast5 files to fastq files during sequencing was also.....
Document: A total of 452 ng viral DNA was used for MinION sequencing. The MinION library was constructed with the Rapid Sequencing Kit (SQK-RAD004) according to the manufacturer's instructions. The library was loaded to a SpotON Flow Cell (FLO-MIN106). Then the control software MinKNOW (v3.1.19, downloaded from the ONT website with a custom account) was set up with a run time of 48 h. The basecalling of fast5 files to fastq files during sequencing was also turned on.
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