Selected article for: "reference database and sequence hit"
Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers
  • Document date: 2020_4_7
    • ID: 18b2foud_18
    Snippet: Centrifuge (v1.0.4) (Kim et al., 2016) aligned the MinION reads to a database composed of human and viral reference genomes to obtain a preliminary summary of target viruses. Minimap2 and Samtools were then used to extract reads aligned to corresponding reference genomes. Canu (v1.8) (Koren et al., 2017) performed de novo assembly of selected reads to generate a draft genome sequence and Nanopolish (v0.11.0) (Loman et al., 2015) was then used to .....
    Document: Centrifuge (v1.0.4) (Kim et al., 2016) aligned the MinION reads to a database composed of human and viral reference genomes to obtain a preliminary summary of target viruses. Minimap2 and Samtools were then used to extract reads aligned to corresponding reference genomes. Canu (v1.8) (Koren et al., 2017) performed de novo assembly of selected reads to generate a draft genome sequence and Nanopolish (v0.11.0) (Loman et al., 2015) was then used to polish the draft sequence. Online BLASTN analysis was conducted to get the best hit of the draft genome sequence.

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