Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_35
Snippet: As the universal PCR primers for HAdV rather than other respiratory pathogens were tested positive, we mainly focused on viral detection from the nanopore reads. A rapid taxonomic analysis revealed that the top four taxonomic ranks with the highest number of unique reads against the NCBI refseq genomes were Homo sapiens (Taxonomy ID = 9606), human adenovirus 7 (Taxonomy ID = 10519), human mastadenovirus B (Taxonomy ID = 108098), and simian adenov.....
Document: As the universal PCR primers for HAdV rather than other respiratory pathogens were tested positive, we mainly focused on viral detection from the nanopore reads. A rapid taxonomic analysis revealed that the top four taxonomic ranks with the highest number of unique reads against the NCBI refseq genomes were Homo sapiens (Taxonomy ID = 9606), human adenovirus 7 (Taxonomy ID = 10519), human mastadenovirus B (Taxonomy ID = 108098), and simian adenovirus 21 (Taxonomy ID = 198503). As the three identified AdVs all belong to the AdV species B, we speculated that the target virus should have high homology with members from this species.
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