Author: Magold, Alexandra I.; Cacquevel, Matthias; Fraering, Patrick C.
Title: Gene Expression Profiling in Cells with Enhanced ?-Secretase Activity Document date: 2009_9_18
ID: 0p8lk12m_42
Snippet: Total RNA was isolated with the RNeasy mini kit following the manufacturer's protocol for adherent cells in the case of CHO cell cultures. For the isolation of total RNA from brain tissue, the TRIzol reagent was used as described in the human samples section. RNA was dissolved in water, which was followed by ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA) quantification and pico chip quality control analysis (6000 Nanochip assa.....
Document: Total RNA was isolated with the RNeasy mini kit following the manufacturer's protocol for adherent cells in the case of CHO cell cultures. For the isolation of total RNA from brain tissue, the TRIzol reagent was used as described in the human samples section. RNA was dissolved in water, which was followed by ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, USA) quantification and pico chip quality control analysis (6000 Nanochip assay Agilent Technologies, Meno Park, USA).
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