Author: Lindqvist, Richard; Mundt, Filip; Gilthorpe, Jonathan D.; Wölfel, Silke; Gekara, Nelson O.; Kröger, Andrea; Överby, Anna K.
Title: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects Document date: 2016_10_24
ID: 09tcnsxv_20
Snippet: Primary WT and IFNAR −/− astrocytes were seeded at 200,000 cells per 12-well. Upon reaching confluence, WT cells were either stimulated with β-PL-inactivated supernatant from WT astrocytes (24 hpi), 5000 U IFNαB/D [49] , or left untreated. Six hours after stimulation, total RNA was isolated using Nucleospin RNA II kit (Macherey-Nagel) according to the manufacturers' instructions. One thousand to three thousand nanograms of RNA was sent to G.....
Document: Primary WT and IFNAR −/− astrocytes were seeded at 200,000 cells per 12-well. Upon reaching confluence, WT cells were either stimulated with β-PL-inactivated supernatant from WT astrocytes (24 hpi), 5000 U IFNαB/D [49] , or left untreated. Six hours after stimulation, total RNA was isolated using Nucleospin RNA II kit (Macherey-Nagel) according to the manufacturers' instructions. One thousand to three thousand nanograms of RNA was sent to GATC Biotech (Konstanz, Germany) for RNAseq (InView TM Transcriptome Explore).
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