Author: Funaro, Ada; Gribaudo, Giorgio; Luganini, Anna; Ortolan, Erika; Lo Buono, Nicola; Vicenzi, Elisa; Cassetta, Luca; Landolfo, Santo; Buick, Richard; Falciola, Luca; Murphy, Marianne; Garotta, Gianni; Malavasi, Fabio
Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells Document date: 2008_11_12
ID: 1u2fkwx3_17
Snippet: Clone stability was tested by maintaining the cells for 3 months in serum-free medium without CpG2006 or other additives, and the mAbs recovered in the supernatant at a concentration of 20-50 μg/ml. All purified IgG maintained their neutralizing activity; mAb 26A1 demonstrated a potent neutralizing capacity, with a calculated inhibitory concentration 50 (IC 50 ) of 0.92 μg/ml against AD169 in HELF (Fig. 3A) , and of 1 μg/ml against VR1814 in .....
Document: Clone stability was tested by maintaining the cells for 3 months in serum-free medium without CpG2006 or other additives, and the mAbs recovered in the supernatant at a concentration of 20-50 μg/ml. All purified IgG maintained their neutralizing activity; mAb 26A1 demonstrated a potent neutralizing capacity, with a calculated inhibitory concentration 50 (IC 50 ) of 0.92 μg/ml against AD169 in HELF (Fig. 3A) , and of 1 μg/ml against VR1814 in HUVEC (Fig. 3B) . The potency of 26A1 against both HCMV strains was ~20 fold higher than the reference anti-HCMV IgG (IC 50 of 16 μg/ ml), currently used in transplant recipients. The inhibitory activity of mAb 26A1 against the whole viral replicative cycle was confirmed by plaque reduction neutralization assay ( Figure 3C ). The mechanism of virus neutralization is subject of ongoing studies.
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