Selected article for: "ac expression significant decrease and wild type"
Author: Barnard, Karen N.; Wasik, Brian R.; LaClair, Justin R.; Buchholz, David W.; Weichert, Wendy S.; Alford-Lawrence, Brynn K.; Aguilar, Hector C.; Parrish, Colin R.
Title: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses
  • Document date: 2019_12_3
    • ID: 11ejfiwe_13
    Snippet: ® This agrees with previous findings that loss of CasD1 leads to loss of both 7,9-O-and 9-O-Ac modifications in haploid HAP1 cells (7) . HPLC analysis of total Sia showed a significant decrease in 9-O-Ac expression compared to wild-type (WT) cells; however, very low levels (Ͻ1%) were still detectable, despite a lack of staining with the HE-Fc probes (Fig. 4E ). This could be due to exogenous Sia from the fetal bovine serum in the growth media, .....
    Document: ® This agrees with previous findings that loss of CasD1 leads to loss of both 7,9-O-and 9-O-Ac modifications in haploid HAP1 cells (7) . HPLC analysis of total Sia showed a significant decrease in 9-O-Ac expression compared to wild-type (WT) cells; however, very low levels (Ͻ1%) were still detectable, despite a lack of staining with the HE-Fc probes (Fig. 4E ). This could be due to exogenous Sia from the fetal bovine serum in the growth media, as is the case for Neu5Gc, which is also detectable at similarly low levels on cells even though the gene for Neu5Gc synthesis, CMAH, is not functional in humans or canines (Fig. S1 ) (46, 47) .

    Search related documents:
    Co phrase search for related documents
    • growth medium and hplc analysis: 1
    • growth medium and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • growth medium and WT cell: 1
    • HAP1 cell and haploid HAP1 cell: 1
    • hplc analysis and low level: 1, 2
    • hplc analysis and significant decrease: 1
    • hplc analysis and total Sia hplc analysis: 1
    • hplc analysis and wild type: 1
    • hplc analysis and WT cell: 1
    • low level and significant decrease: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • low level and similarly low level: 1, 2
    • low level and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • previous finding and significant decrease: 1
    • previous finding and wild type: 1, 2, 3
    • significant decrease and total Sia hplc analysis: 1
    • significant decrease and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • total Sia hplc analysis and wild type: 1
    • wild type and WT cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
    • wild type WT cell and WT cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23