Selected article for: "bovine serum and wild type"
Author: Barnard, Karen N.; Wasik, Brian R.; LaClair, Justin R.; Buchholz, David W.; Weichert, Wendy S.; Alford-Lawrence, Brynn K.; Aguilar, Hector C.; Parrish, Colin R.
Title: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses
  • Document date: 2019_12_3
    • ID: 11ejfiwe_13
    Snippet: ® This agrees with previous findings that loss of CasD1 leads to loss of both 7,9-O-and 9-O-Ac modifications in haploid HAP1 cells (7) . HPLC analysis of total Sia showed a significant decrease in 9-O-Ac expression compared to wild-type (WT) cells; however, very low levels (Ͻ1%) were still detectable, despite a lack of staining with the HE-Fc probes (Fig. 4E ). This could be due to exogenous Sia from the fetal bovine serum in the growth media, .....
    Document: ® This agrees with previous findings that loss of CasD1 leads to loss of both 7,9-O-and 9-O-Ac modifications in haploid HAP1 cells (7) . HPLC analysis of total Sia showed a significant decrease in 9-O-Ac expression compared to wild-type (WT) cells; however, very low levels (Ͻ1%) were still detectable, despite a lack of staining with the HE-Fc probes (Fig. 4E ). This could be due to exogenous Sia from the fetal bovine serum in the growth media, as is the case for Neu5Gc, which is also detectable at similarly low levels on cells even though the gene for Neu5Gc synthesis, CMAH, is not functional in humans or canines (Fig. S1 ) (46, 47) .

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