Selected article for: "HDV Hepatitis delta virus and Hepatitis delta"
Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension
  • Document date: 2018_12_6
    • ID: 012ipcdr_28
    Snippet: The pUC19-HH-pre-let-7a-1-HDV vector used for in vitro transcription of pre-let-7a-1 was constructed using a pUC19 vector, which allows for synthesis of pre-let-7a-1 flanked by a 5' Hammerhead ribozyme (HH) and a 3' Hepatitis delta virus (HDV) ribozyme. Plasmids were amplified in E. coli DH5α cells grown in LB medium supplemented with 100 mg/L ampicillin and purified using the QIAGEN Plasmid Maxi Kit. Plasmids were linearized with HindIII (New E.....
    Document: The pUC19-HH-pre-let-7a-1-HDV vector used for in vitro transcription of pre-let-7a-1 was constructed using a pUC19 vector, which allows for synthesis of pre-let-7a-1 flanked by a 5' Hammerhead ribozyme (HH) and a 3' Hepatitis delta virus (HDV) ribozyme. Plasmids were amplified in E. coli DH5α cells grown in LB medium supplemented with 100 mg/L ampicillin and purified using the QIAGEN Plasmid Maxi Kit. Plasmids were linearized with HindIII (New England Biolabs) and stored as is at − 20°C at a concentration of 1 mg/mL. All plasmids were verified by DNA sequencing.

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