Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension Document date: 2018_12_6
ID: 012ipcdr_32
Snippet: For optimization of transfection conditions, small-scale cultures were used: 2-mL cultures were grown in 6-well plates (Starstedt #83.1839.500) and 20-mL cultures were grown in 125-mL disposable Erlenmeyer flasks (Corning #431143). For Dicer purification, large-scale cultures of 150 mL to 1 L were grown in thoroughly rinsed and sterilized 1-L glass Erlenmeyer (Corning #49851 L) or 2.8-L Fernbach culture flasks (Corning #44262×L). The incubation .....
Document: For optimization of transfection conditions, small-scale cultures were used: 2-mL cultures were grown in 6-well plates (Starstedt #83.1839.500) and 20-mL cultures were grown in 125-mL disposable Erlenmeyer flasks (Corning #431143). For Dicer purification, large-scale cultures of 150 mL to 1 L were grown in thoroughly rinsed and sterilized 1-L glass Erlenmeyer (Corning #49851 L) or 2.8-L Fernbach culture flasks (Corning #44262×L). The incubation time after transfection was fixed to 72 h for large-scale cultures.
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