Author: Funaro, Ada; Gribaudo, Giorgio; Luganini, Anna; Ortolan, Erika; Lo Buono, Nicola; Vicenzi, Elisa; Cassetta, Luca; Landolfo, Santo; Buick, Richard; Falciola, Luca; Murphy, Marianne; Garotta, Gianni; Malavasi, Fabio
Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells Document date: 2008_11_12
ID: 1u2fkwx3_18
Snippet: The broad and robust neutralizing activity of mAb 26A1 encouraged us to generate recombinant IgG suitable as prophylactic or therapeutic tools in clinical applications. To this aim, cells were submitted for 5' RACE PCR amplification, the PCR products of two amplification reactions were cloned using a Eco RI restriction site in a sequencing vector and used for transforming TOP10 E. coli. A single VH and VL sequence was obtained from each cell cult.....
Document: The broad and robust neutralizing activity of mAb 26A1 encouraged us to generate recombinant IgG suitable as prophylactic or therapeutic tools in clinical applications. To this aim, cells were submitted for 5' RACE PCR amplification, the PCR products of two amplification reactions were cloned using a Eco RI restriction site in a sequencing vector and used for transforming TOP10 E. coli. A single VH and VL sequence was obtained from each cell culture, confirming that each original culture was clonal ( Figure 4 ).
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