Author: Lindqvist, Richard; Mundt, Filip; Gilthorpe, Jonathan D.; Wölfel, Silke; Gekara, Nelson O.; Kröger, Andrea; Överby, Anna K.
Title: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects Document date: 2016_10_24
ID: 09tcnsxv_24
Snippet: Cells were incubated with DMEM, 10 % heat-inactivated FBS, and 2 mM glutamine with MAR1-5A3 antibody (Affymetrix eBioscience, 16-5945-85 [19] ) or IgG1 κ isotype control (eBioscience, 14-4714-85) at a concentration of 10 μg/mL for 2 h at 37°C. After 2 h, the antibodies were removed, and cells were infected with TBEV for 1 h. Following removal of the viral infection inoculum, the medium containing 10 μg/mL antibody was returned to the cells......
Document: Cells were incubated with DMEM, 10 % heat-inactivated FBS, and 2 mM glutamine with MAR1-5A3 antibody (Affymetrix eBioscience, 16-5945-85 [19] ) or IgG1 κ isotype control (eBioscience, 14-4714-85) at a concentration of 10 μg/mL for 2 h at 37°C. After 2 h, the antibodies were removed, and cells were infected with TBEV for 1 h. Following removal of the viral infection inoculum, the medium containing 10 μg/mL antibody was returned to the cells.
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