Author: Huang, Nan-Chieh; Hung, Wan-Ting; Tsai, Wei-Lun; Lai, Feng-Yi; Lin, You-Sheng; Huang, Mei-Shu; Chen, Jih-Jung; Lin, Wei-Yu; Weng, Jing-Ru; Chang, Tsung-Hsien
Title: Ficus septica plant extracts for treating Dengue virus in vitro Document date: 2017_6_8
ID: 1a3cmi9i_11
Snippet: In the extracts screening of DENV inhibition, the cells (5×10 3 cells) were treated with plant extracts with serial dilution dose of 100, 50, 25, 12.5, 6.25, 3.125 or 1.56 µg/ml or DMSO solvent control for 3 h. Then, these cells were infected by DENV-1, DENV-2 and AiV infection (multiplicity of infection [MOI] = 2.5). After 2 h virus adsorption, the medium mixture was replaced by fresh growth medium. At 42 h after infection, the virus-infected .....
Document: In the extracts screening of DENV inhibition, the cells (5×10 3 cells) were treated with plant extracts with serial dilution dose of 100, 50, 25, 12.5, 6.25, 3.125 or 1.56 µg/ml or DMSO solvent control for 3 h. Then, these cells were infected by DENV-1, DENV-2 and AiV infection (multiplicity of infection [MOI] = 2.5). After 2 h virus adsorption, the medium mixture was replaced by fresh growth medium. At 42 h after infection, the virus-infected cells were analyzed by immunofluorescence assay. In another experiment, the viral stocks of DENV-1, DENV-2 and AiV were preincubated with a series of dilution doses of leaf methanol extract of F. septica (FS-(L)-M) for 1 h at room temperature. The mixture of virus plus the plant extract was then used to infect A549 cells.
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