Selected article for: "previously RT PCR and RT PCR"
Author: Franzo, Giovanni; Cecchinato, Mattia; Tosi, Giovanni; Fiorentini, Laura; Faccin, Francesca; Tucciarone, Claudia Maria; Trogu, Tiziana; Barbieri, Ilaria; Massi, Paola; Moreno, Ana
Title: GI-16 lineage (624/I or Q1), there and back again: The history of one of the major threats for poultry farming of our era
  • Document date: 2018_12_20
    • ID: 0pi042qi_2
    Snippet: Viral RNA was extracted from isolates using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. IBV was detected using RT-PCR as previously described [25] . Partial S1 gene (464-bp), covering the hypervariable region 3 (HVR3), was performed using the same primers that were used for RT-PCR. The obtained chromatogram quality was evaluated by FinchTV (http://www.geospiza.com) and the consensus sequences were .....
    Document: Viral RNA was extracted from isolates using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. IBV was detected using RT-PCR as previously described [25] . Partial S1 gene (464-bp), covering the hypervariable region 3 (HVR3), was performed using the same primers that were used for RT-PCR. The obtained chromatogram quality was evaluated by FinchTV (http://www.geospiza.com) and the consensus sequences were reconstructed using CromasPro (CromasPro Version 1.5).

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