Author: Cong, Wei; Zhang, Xiao-Xuan; He, Jun-Jun; Li, Fa-Cai; Elsheikha, Hany M.; Zhu, Xing-Quan
Title: Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection Document date: 2017_3_10
ID: 05tshufp_33
Snippet: The available software miREvo [57] and mirdeep2 [56] were integrated to predict novel miRNA through exploring the secondary structure, the dicer cleavage site and the minimum free energy of the small RNA tags unannotated in the former steps. miRNA expression levels were estimated with TPM (transcript per million) units [58] . Differential expression analysis of infected versus control groups was performed using the DESeq R package (1.8.3). The P-.....
Document: The available software miREvo [57] and mirdeep2 [56] were integrated to predict novel miRNA through exploring the secondary structure, the dicer cleavage site and the minimum free energy of the small RNA tags unannotated in the former steps. miRNA expression levels were estimated with TPM (transcript per million) units [58] . Differential expression analysis of infected versus control groups was performed using the DESeq R package (1.8.3). The P-values was adjusted using the Benjamini & Hochberg method. Corrected P-value of 0.05 was set as the threshold for significantly differential expression by default.
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