Author: Lu, Shuai; Chen, Yingzhu; Qin, Kun; Zhou, Jianfang; Lou, Yongliang; Tan, Wenjie
Title: Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China Document date: 2016_4_15
ID: 1ghgd7yg_12
Snippet: Since the N proteins of coronaviruses are relatively conserved, we aimed to determine whether the N protein was responsible for antigenic cross-reactivity. Western blot analysis showed that the CCoV N protein cross-reacted weakly with mice antisera against the NP of HCoV-NL63 and 229E, indicating the N protein of CCoV may share the same antigenic epitopes with that of human alphacoronaviruses. However, the CRCoV N protein can react strongly with .....
Document: Since the N proteins of coronaviruses are relatively conserved, we aimed to determine whether the N protein was responsible for antigenic cross-reactivity. Western blot analysis showed that the CCoV N protein cross-reacted weakly with mice antisera against the NP of HCoV-NL63 and 229E, indicating the N protein of CCoV may share the same antigenic epitopes with that of human alphacoronaviruses. However, the CRCoV N protein can react strongly with mice polyclonal sera against NP of HCoV-HKU1, OC43, NL63 and 229E, while having no reactivity with that of MERS-CoV ( Figure 5 ).
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