Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_12
Snippet: Total DNA was sheared into ∼350 bp fragments by the Covaris ultrasonicator (Covaris, Woburn, MA, United States). A-tailed, ligated to paired-end adaptors and PCR amplified with a 350 bp insert were used for the library construction. Qubit 2.0 (Life Technologies) was then used to quantify the concentration of the library, and the insert size was tested with Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, United States). Illumina NovaSeq 6000.....
Document: Total DNA was sheared into ∼350 bp fragments by the Covaris ultrasonicator (Covaris, Woburn, MA, United States). A-tailed, ligated to paired-end adaptors and PCR amplified with a 350 bp insert were used for the library construction. Qubit 2.0 (Life Technologies) was then used to quantify the concentration of the library, and the insert size was tested with Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, United States). Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, United States) was used to generate 2 × 150 bp pair-end reads.
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