Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_19
Snippet: In order to investigate the minimal read number sufficient to identify present isolate, we downsampled the nanopore reads by randomly extracting reads at specific sequencing depths of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400 , 500, 1,000, 5,000, 10,000, 15,000, 20,000, 25,000, 30,000, and 35,000 for 1,000 times. The reads were aligned to the MinION genome sequence by Minimap2, and the alignment statistics were summarized by using.....
Document: In order to investigate the minimal read number sufficient to identify present isolate, we downsampled the nanopore reads by randomly extracting reads at specific sequencing depths of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400 , 500, 1,000, 5,000, 10,000, 15,000, 20,000, 25,000, 30,000, and 35,000 for 1,000 times. The reads were aligned to the MinION genome sequence by Minimap2, and the alignment statistics were summarized by using Samtools and Perl scripts.
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