First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers

Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai

and a 45 bp deletion were detected in the penton protein, and one 3 bp deletion and one 15 bp insertion were found in 100 kDa hexon assembly associated protein, which are shared features in subtype 21a (Hage et al., 2014) and 21b relative to the prototypes. Notably, some deletions and insertions occur in the intergeneric regions of CDSs; that is, an insertion of 6-12 bp (all As) between penton protein and protein VII precursor was detected in the present genome relative to all other references. Moreover, a deletion of 9 bp between protein VI precursor and hexon protein was found in comparisons of the current genome with the prototypes. Multiple polymorphisms were also discovered in the non-annotated 5 region of the genome, such as a 1 bp deletion at position 242 and a conversion of C to T at position 252, the effects of which on viral life need further investigation.