Selected article for: "PCR testing and real time"
Author: Vittecoq, Marion; Grandhomme, Viviane; Champagnon, Jocelyn; Guillemain, Matthieu; Crescenzo-Chaigne, Bernadette; Renaud, François; Thomas, Frédéric; Gauthier-Clerc, Michel; van der Werf, Sylvie
Title: High Influenza A Virus Infection Rates in Mallards Bred for Hunting in the Camargue, South of France
  • Document date: 2012_8_27
    • ID: 0r4z1zea_8
    Snippet: The infection rates in the different GBF were determined by real-time RT-PCR targeting the conserved M gene of AIV (Table 1) . A very high infection rate (99%) was observed in the single GBF sampled in 2009. In 2010 the infection rates ranged between 0 and 24%, being of 8% in the farm infected in 2009. Initial subtyping, searching for H5, H7, H9, N1, and N7 by realtime RT-PCR performed on positive samples led to the identification of an H10N7 str.....
    Document: The infection rates in the different GBF were determined by real-time RT-PCR targeting the conserved M gene of AIV (Table 1) . A very high infection rate (99%) was observed in the single GBF sampled in 2009. In 2010 the infection rates ranged between 0 and 24%, being of 8% in the farm infected in 2009. Initial subtyping, searching for H5, H7, H9, N1, and N7 by realtime RT-PCR performed on positive samples led to the identification of an H10N7 strain in the GBF in 2009 and further testing for H10 by real-time RT-PCR showed that the outbreak involved a single H10N7 virus (named H10N7 Camargue below). The viral strains involved in the infections observed in 2010 were LPAIV. H5, H7, H9, H10, and N7 subtypes were searched among them but none was detected.

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