Author: Vidaña, Beatriz; Martínez, Jorge; Martínez-Orellana, Pamela; García Migura, Lourdes; Montoya, María; Martorell, Jaime; Majó, Natàlia
Title: Heterogeneous pathological outcomes after experimental pH1N1 influenza infection in ferrets correlate with viral replication and host immune responses in the lung Document date: 2014_8_28
ID: 0hyg403m_24
Snippet: Apoptotic cells in the lungs were also detected by IHC using the anti-caspase 3 polyclonal antibody (Cell Signalling, Cleaved Caspase-3 (Asp175) Antibody, ref 9661) diluted 1:100 in PBS using Ethylenediaminetetraacetic acid (EDTA) as the antigen retrieval method. The IHC technique was performed as described above for inflammatory cell immunophenotyping. Swine and ferret lymph node sections were used as positive controls, and the sections in which.....
Document: Apoptotic cells in the lungs were also detected by IHC using the anti-caspase 3 polyclonal antibody (Cell Signalling, Cleaved Caspase-3 (Asp175) Antibody, ref 9661) diluted 1:100 in PBS using Ethylenediaminetetraacetic acid (EDTA) as the antigen retrieval method. The IHC technique was performed as described above for inflammatory cell immunophenotyping. Swine and ferret lymph node sections were used as positive controls, and the sections in which the specific primary antibodies were substituted with PBS were used as negative controls.
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