Author: Aran Singanayagam; Su-Ling Loo; Maria Calderazzo; Lydia J Finney; Maria-Belen Trujillo Torralbo; Eteri Bakhsoliani; Jason Girkin; Punnam Veerati; Prabuddha S Pathinayake; Kristy S Nichol; Andrew Reid; Joseph Foottit; Sebastian L Johnston; Nathan W Bartlett; Patrick Mallia
Title: Anti-microbial immunity is impaired in COPD patients with frequent exacerbations Document date: 2019_5_9
ID: 72jzqm86_17
Snippet: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2µg was used for cDNA synthesis (Omniscript RT kit). Quantitative PCR was carried out using previously described specific primers and probes for each gene of interest 23 , and normalized to 18S rRNA. Reactions were analysed using ABI 7500 Fast Realtime PCR system (Applied Biosystems, Carlsbad, CA). author/funder. Al.....
Document: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2µg was used for cDNA synthesis (Omniscript RT kit). Quantitative PCR was carried out using previously described specific primers and probes for each gene of interest 23 , and normalized to 18S rRNA. Reactions were analysed using ABI 7500 Fast Realtime PCR system (Applied Biosystems, Carlsbad, CA). author/funder. All rights reserved. No reuse allowed without permission.
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