Author: Cong, Wei; Zhang, Xiao-Xuan; He, Jun-Jun; Li, Fa-Cai; Elsheikha, Hany M.; Zhu, Xing-Quan
Title: Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection Document date: 2017_3_10
ID: 05tshufp_27
Snippet: Genomic DNA was extracted from the collected liver samples using TIANamp Genomic DNA kit according to the manufacturer's recommendations (TianGenâ„¢, Beijing, China). Then, a semi-nested PCR targeting T. gondii B1 gene was performed to detect T. gondii infection [53] . DNA samples giving positive B1 amplification were genotyped using PCR-restriction fragment length polymorphism analysis as described previously [54] ......
Document: Genomic DNA was extracted from the collected liver samples using TIANamp Genomic DNA kit according to the manufacturer's recommendations (TianGenâ„¢, Beijing, China). Then, a semi-nested PCR targeting T. gondii B1 gene was performed to detect T. gondii infection [53] . DNA samples giving positive B1 amplification were genotyped using PCR-restriction fragment length polymorphism analysis as described previously [54] .
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