Author: Izquierdo, Laure; Oliveira, Catarina; Fournier, Carole; Descamps, Véronique; Morel, Virginie; Dubuisson, Jean; Brochot, Etienne; Francois, Catherine; Castelain, Sandrine; Duverlie, Gilles; Helle, Francois
Title: Hepatitis C Virus Resistance to Carbohydrate-Binding Agents Document date: 2016_2_12
ID: 1a4l1beo_19
Snippet: We used the QuickChange II XL Site Directed Mutagenesis Kit (Agilent Technologies) to introduce the potential resistance mutations in the plasmid encoding the JFH1-CS-A4-Rluc-DM genome [17] , noted WT in this study. The nucleotide sequences of each mutant were verified. Replication and infectivity were assessed by measuring Renilla Luciferase activities using the Renilla Luciferase Assay System (Promega) and a Berthold CentroXS3 LB 960 luminomete.....
Document: We used the QuickChange II XL Site Directed Mutagenesis Kit (Agilent Technologies) to introduce the potential resistance mutations in the plasmid encoding the JFH1-CS-A4-Rluc-DM genome [17] , noted WT in this study. The nucleotide sequences of each mutant were verified. Replication and infectivity were assessed by measuring Renilla Luciferase activities using the Renilla Luciferase Assay System (Promega) and a Berthold CentroXS3 LB 960 luminometer.
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