Selected article for: "control group and standard protocol"
Author: Vidaña, Beatriz; Martínez, Jorge; Martínez-Orellana, Pamela; García Migura, Lourdes; Montoya, María; Martorell, Jaime; Majó, Natàlia
Title: Heterogeneous pathological outcomes after experimental pH1N1 influenza infection in ferrets correlate with viral replication and host immune responses in the lung
  • Document date: 2014_8_28
    • ID: 0hyg403m_11
    Snippet: The animals were divided into three groups. The control group included two ferrets that were inoculated intratracheally with 0.1 M phosphate-buffered saline (pH = 7.5) (PBS). The R61 group included 4 ferrets that were infected with the R61 virus, and the R11 group included 4 ferrets that were infected with the R11 virus. The ferrets were intratracheally inoculated with 10 6 TCID 50 /mL of the appropriate virus, and necropsies were performed on da.....
    Document: The animals were divided into three groups. The control group included two ferrets that were inoculated intratracheally with 0.1 M phosphate-buffered saline (pH = 7.5) (PBS). The R61 group included 4 ferrets that were infected with the R61 virus, and the R11 group included 4 ferrets that were infected with the R11 virus. The ferrets were intratracheally inoculated with 10 6 TCID 50 /mL of the appropriate virus, and necropsies were performed on days 4 and 7 postinfection. The animals were euthanized by intravenous injection of sodium pentobarbital (100 mg/kg) under anaesthesia with ketamine (5-10 mg/kg) (Imalgene 1000® Merial, S.A., Spain) and medetomidine (0.05 mg/kg) (Domtor® Pfiser, S.A., Spain) and were then necropsied according to a standard protocol.

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