Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension Document date: 2018_12_6
ID: 012ipcdr_33
Snippet: At harvest time, cells were first counted and then pelleted by centrifugation at 200×g and washed 3 times with at least 5-pellet volumes of cold PBS. The washed cell pellet was either used immediately or frozen in liquid nitrogen and stored at − 80°C for later use......
Document: At harvest time, cells were first counted and then pelleted by centrifugation at 200×g and washed 3 times with at least 5-pellet volumes of cold PBS. The washed cell pellet was either used immediately or frozen in liquid nitrogen and stored at − 80°C for later use.
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