Selected article for: "Extraction kit and real time polymerase"
Author: Toh, Teck-Hock; Hii, King-Ching; Fieldhouse, Jane K; Ting, Jakie; Berita, Antoinette; Nguyen, Tham Thi; Wong, See-Chang; Wong, Toh-Mee; Lim, Wei-Honn; Ha, Siaw-Jing; Lau, Chuet-Zou; Kong, Sing-Ling; Bailey, Emily S; Warkentien, Tyler E; Husain, Tupur S; Gray, Gregory C
Title: High Prevalence of Viral Infections Among Hospitalized Pneumonia Patients in Equatorial Sarawak, Malaysia
  • Document date: 2019_2_13
    • ID: 08kqtw8s_14
    Snippet: All specimens were stored at −80°C at the Sibu Hospital Clinical Research Center (SHCRC) until ribonucleic acid or deoxyribonucleic acid extraction was performed using the QIAmp Cador Pathogen Mini Kit (QIAGEN, Hilden, Germany). Specimens collected in Kapit were stored at −80°C until they could be transported to Sibu for processing. Real-time reverse-transcription polymerase chain reaction (rRT-PCR) or real-time PCR (rPCR) was conducted on .....
    Document: All specimens were stored at −80°C at the Sibu Hospital Clinical Research Center (SHCRC) until ribonucleic acid or deoxyribonucleic acid extraction was performed using the QIAmp Cador Pathogen Mini Kit (QIAGEN, Hilden, Germany). Specimens collected in Kapit were stored at −80°C until they could be transported to Sibu for processing. Real-time reverse-transcription polymerase chain reaction (rRT-PCR) or real-time PCR (rPCR) was conducted on similar BioRad CFx96 C1000 Touch Thermal Cycler Real-Time systems at SHCRC, Duke-NUS in Singapore and at Duke University in Durham, North Carolina.

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