Author: Toh, Teck-Hock; Hii, King-Ching; Fieldhouse, Jane K; Ting, Jakie; Berita, Antoinette; Nguyen, Tham Thi; Wong, See-Chang; Wong, Toh-Mee; Lim, Wei-Honn; Ha, Siaw-Jing; Lau, Chuet-Zou; Kong, Sing-Ling; Bailey, Emily S; Warkentien, Tyler E; Husain, Tupur S; Gray, Gregory C
Title: High Prevalence of Viral Infections Among Hospitalized Pneumonia Patients in Equatorial Sarawak, Malaysia Document date: 2019_2_13
ID: 08kqtw8s_15
Snippet: All NP swabs were examined for molecular evidence of influenza A (IAV), IBV, ICV, and IDV, human adenovirus (AdV), human enterovirus (EV), human coronavirus (CoV), RSV subtype A (RSV-A) and RSV-B, and PIV types 1-4 (PIV-1, -2, -3, and -4). Primer and probes sequences for targeted pathogens are recorded in Supplementary Table 2. A positive control and a no-template control were included in each run. All specimens were first run at SHCRC. One-milli.....
Document: All NP swabs were examined for molecular evidence of influenza A (IAV), IBV, ICV, and IDV, human adenovirus (AdV), human enterovirus (EV), human coronavirus (CoV), RSV subtype A (RSV-A) and RSV-B, and PIV types 1-4 (PIV-1, -2, -3, and -4). Primer and probes sequences for targeted pathogens are recorded in Supplementary Table 2. A positive control and a no-template control were included in each run. All specimens were first run at SHCRC. One-milliliter aliquots of these specimens were later shipped on dry ice to either Duke-NUS or Duke University for validation. Laboratory staff at Duke-NUS and Duke were blind to the results of the assays at SHCRC. Cycle threshold (Ct) values <38 were considered positive, and Ct values 38 to 40 were considered suspect to acknowledge that positivity could be the result of cross-reactivity or nonspecific amplification. Cycle threshold values >40 were considered negative. All discrepant assays were noted and repeated at both SHCRC and either Duke or Duke-NUS, using the same original assays, and discrepancies were thus resolved. Partial genome sequencing was also performed for several specimens to validate discrepant results.
Search related documents:
Co phrase search for related documents- adv human adenovirus and cross reactivity: 1
- adv human adenovirus and Ct value: 1, 2, 3
- adv human adenovirus and cycle threshold: 1, 2, 3, 4, 5
- adv human adenovirus and EV human enterovirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- adv human adenovirus and human adenovirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- adv human adenovirus and human enterovirus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- adv human adenovirus and molecular evidence: 1
- adv human adenovirus and nonspecific amplification: 1
- adv human adenovirus and piv type: 1, 2, 3
- adv human adenovirus and RSV subtype: 1, 2
- assay result and cross reactivity: 1, 2, 3, 4, 5
- assay result and Ct value: 1, 2
- assay result and cycle threshold: 1, 2, 3, 4
- assay result and discrepant assay: 1
- assay result and genome sequencing: 1
- assay result and human adenovirus: 1
- assay result and positive control: 1, 2, 3, 4
- assay result and RSV subtype: 1
- CoV human coronavirus EV human enterovirus and EV human enterovirus: 1, 2
Co phrase search for related documents, hyperlinks ordered by date