Selected article for: "cycle threshold and positive control"
Author: Toh, Teck-Hock; Hii, King-Ching; Fieldhouse, Jane K; Ting, Jakie; Berita, Antoinette; Nguyen, Tham Thi; Wong, See-Chang; Wong, Toh-Mee; Lim, Wei-Honn; Ha, Siaw-Jing; Lau, Chuet-Zou; Kong, Sing-Ling; Bailey, Emily S; Warkentien, Tyler E; Husain, Tupur S; Gray, Gregory C
Title: High Prevalence of Viral Infections Among Hospitalized Pneumonia Patients in Equatorial Sarawak, Malaysia
  • Document date: 2019_2_13
    • ID: 08kqtw8s_15
    Snippet: All NP swabs were examined for molecular evidence of influenza A (IAV), IBV, ICV, and IDV, human adenovirus (AdV), human enterovirus (EV), human coronavirus (CoV), RSV subtype A (RSV-A) and RSV-B, and PIV types 1-4 (PIV-1, -2, -3, and -4). Primer and probes sequences for targeted pathogens are recorded in Supplementary Table 2. A positive control and a no-template control were included in each run. All specimens were first run at SHCRC. One-milli.....
    Document: All NP swabs were examined for molecular evidence of influenza A (IAV), IBV, ICV, and IDV, human adenovirus (AdV), human enterovirus (EV), human coronavirus (CoV), RSV subtype A (RSV-A) and RSV-B, and PIV types 1-4 (PIV-1, -2, -3, and -4). Primer and probes sequences for targeted pathogens are recorded in Supplementary Table 2. A positive control and a no-template control were included in each run. All specimens were first run at SHCRC. One-milliliter aliquots of these specimens were later shipped on dry ice to either Duke-NUS or Duke University for validation. Laboratory staff at Duke-NUS and Duke were blind to the results of the assays at SHCRC. Cycle threshold (Ct) values <38 were considered positive, and Ct values 38 to 40 were considered suspect to acknowledge that positivity could be the result of cross-reactivity or nonspecific amplification. Cycle threshold values >40 were considered negative. All discrepant assays were noted and repeated at both SHCRC and either Duke or Duke-NUS, using the same original assays, and discrepancies were thus resolved. Partial genome sequencing was also performed for several specimens to validate discrepant results.

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