Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_42
Snippet: Virus infection. The production of CVB3 from infectious clones was described previously (39) . Virus titers were determined by endpoint titration according to the method of Reed and Muench and expressed as 50% cell culture infective doses (CCID 50 ). BGM or HeLa cells were infected with virus for 30 min to 1 h. Following removal of the inoculum, fresh medium was added to the cells. At the time points indicated in the figures, cells were either fi.....
Document: Virus infection. The production of CVB3 from infectious clones was described previously (39) . Virus titers were determined by endpoint titration according to the method of Reed and Muench and expressed as 50% cell culture infective doses (CCID 50 ). BGM or HeLa cells were infected with virus for 30 min to 1 h. Following removal of the inoculum, fresh medium was added to the cells. At the time points indicated in the figures, cells were either fixed for immunolabeling, lysed to measure the amount of viral RNA by quantitative PCR, or frozen to determine the amount of infectious virus particles by titration analysis.
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