Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase

Takenouchi, Takato; Kitani, Hiroshi; Suzuki, Shunichi; Nakai, Michiko; Fuchimoto, Dai-ichiro; Tsukimoto, Mitsutoshi; Shinkai, Hiroki; Sato, Mitsuru; Uenishi, Hirohide

The primary antibodies were as follows: mouse monoclonal anti-cytokeratin 18 (CK18; Millipore); mouse monoclonal anti-cytokeratin 19 (CK19; Progen, Heidelberg, Germany); mouse monoclonal anti-α-smooth muscle actin (SMA; Progen); mouse monoclonal anti-macrophage scavenger receptor A (MSR-A:CD204) (KT022; TransGenic, Inc., Kumamoto, Japan); rabbit polyclonal anti-ionized calcium-binding adaptor molecule 1 (Iba1; Wako); and mouse monoclonal anti-CD172a (VMRD, Inc., Pullman, WA, USA). Since we have already confirmed that the KT022, Iba1, and CD172a antibodies react with antigens expressed by porcine primary macrophages (12, 13) , these antibodies were used as markers of porcine macrophages.