HyperISGylation of Old World Monkey ISG15 in Human Cells

Pattyn, Els; Verhee, Annick; Uyttendaele, Isabel; Piessevaux, Julie; Timmerman, Evy; Gevaert, Kris; Vandekerckhove, Joël; Peelman, Frank; Tavernier, Jan

We isolated ISG15 cDNAs of Humans (allelic variant with N at position 83 and S at position 94) (Homo sapiens, hereafter HuISG15), Chimpanzee (Pan troglodytes, CpzISG15), African Green Monkey (Cercopithecus aethiops, AgmISG15), Rhesus Monkey (Macaca mulatta, RhmISG15) and Mouse (Mus musculus, MoISG15). All were Cterminally cloned after a FLAG recognition tag. HuISG15 and CpzISG15 only differ by one amino acid (respectively a Ser and Asn residue at position 21) and represent hominid ISG15 (HOmISG15). AgmISG15 and RhmISG15 differ from each other by two amino acids (respectively a Ser and Asn residue at position 21 and Arg and Lys at position 77), and are both part of the Old World Monkeys (hereafter referred to as OWmISG15) (see Table 1 ).