Author: Chenoll, Empar; Casinos, Beatriz; Bataller, Esther; Buesa, Javier; Ramón, Daniel; Genovés, Salvador; Fábrega, Joan; Rivero Urgell, Montserrat; Moreno Muñoz, José A.
Title: Identification of a Peptide Produced by Bifidobacterium longum CECT 7210 with Antirotaviral Activity Document date: 2016_5_4
ID: 0sxl6f1r_25
Snippet: The protease assay was performed at different pH values with purified protease. A β-casein solution (2% w/v) was incubated with purified protease at 50 • C (optimum temperature) for 72 h. The hydrolysis reaction was carried out in a solution with purified protease (10 mL), β-casein solution (2% w/v; 20 mL) and PBS buffer (70 mL), at pH 4, 6, 7, 8, and 10. After incubation, samples were boiled for 10 min to stop the reaction, filtered through .....
Document: The protease assay was performed at different pH values with purified protease. A β-casein solution (2% w/v) was incubated with purified protease at 50 • C (optimum temperature) for 72 h. The hydrolysis reaction was carried out in a solution with purified protease (10 mL), β-casein solution (2% w/v; 20 mL) and PBS buffer (70 mL), at pH 4, 6, 7, 8, and 10. After incubation, samples were boiled for 10 min to stop the reaction, filtered through a Millipore 0.45 µm pore-size filter and 11-mer peptide was analyzed by HPLC.
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