Author: Cong, Wei; Zhang, Xiao-Xuan; He, Jun-Jun; Li, Fa-Cai; Elsheikha, Hany M.; Zhu, Xing-Quan
Title: Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection Document date: 2017_3_10
ID: 05tshufp_29
Snippet: Total RNA was prepared individually from the cryopreserved liver tissues of the cats using TRIzol Reagent according to the manufacturer's instructions (Invitrogen Co. Ltd). RNA degradation and contamination was checked on 1% agarose gels. RNA purity was evaluated using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was determined using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA i.....
Document: Total RNA was prepared individually from the cryopreserved liver tissues of the cats using TRIzol Reagent according to the manufacturer's instructions (Invitrogen Co. Ltd). RNA degradation and contamination was checked on 1% agarose gels. RNA purity was evaluated using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was determined using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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